The Scholars
of the WANIDA network
Curriculum :
Molecular Biology and Genetic Engineering
ACE affiliate :
CEA ITECH-MTV
Supervisor :
Pr Macaire OUEDRAOGO, UNB
Co-supervisor :
Pr Abdoulaye DIABATE, CEA ITECH-MTV
Other contributors under PhD/MSc supervision:
Dr Thérèse KAGONE, LNR-FHV/Muraz
Agossi Edwige Kpodo
Curriculum :
Molecular Biology and Genetic Engineering
ACE affiliate :
CEA ITECH-MTV
Supervisor :
Pr Macaire OUEDRAOGO, UNB
Co-supervisor :
Pr Abdoulaye DIABATE, CEA ITECH-MTV
Other contributors under PhD/MSc supervision:
Dr Thérèse KAGONE, LNR-FHV/Muraz
Date of support: 01/01/21 to 28/02/22
Date of Master's defense: 02/04/2022
ORCID profile:
Project title:
Molecular Epidemiology of Dengue Viruses around Outbreaks in Burkina Faso from June to November 2021
Summary of your Master's subject :
Dengue fever, a viral disease of tropical and subtropical regions, is transmitted by female mosquitoes of the genus Aedes, whose virus (DENV) belongs to the Flaviviridae family, genus Flavivirus. There is no specific treatment, but appropriate rehydration can reduce the occurrence of severe forms. Dengue fever is a major public health problem due to its worldwide spread, with epidemic episodes. In order to ensure the epidemiological monitoring and control of this disease, it is crucial to set up an effective virus surveillance system in both human and mosquito populations, which would provide a real warning tool for the early detection of possible epidemics. We determined dengue seroprevalence for the year 2020 on human blood samples collected in different regions of Burkina Faso using IgM ELISA and PCR techniques at the Laboratoire National de Référence des Fièvres Hémorragiques et Virales (LNR-FHV). We then tested field mosquitoes for DENV RNA using RT-qPCR to estimate entomological risk indicators.
Summary of results:
Results: Of five hundred and fifty-seven (557) samples collected, four hundred and seventy-four (474) were analyzed, representing a completion rate of 85%. Distribution by sex and age showed that there were more women (60.55%) than men (39.45%), giving a sex ratio (M/F) of 0.65. Regarding infection by age group, the age group was the most represented, and the highest prevalence was observed in the age group. Seroprevalence of infection was 2.53%, with (1.48%) males positive versus (1.05%) females. In addition, none of the Aedes aegypti pools tested positive for dengue virus in the vector. Dengue seroprevalence was 2.53% in 2020, with sick men outnumbering women. Virus detection in Aedes aegypti in 2021 showed that none of the mosquito pools were infected.
Expected results / Main objective :
Assess the transmission dynamics and epidemic potential of dengue virus serotypes in monitoring the emergence of circulating strains in the Centre and Hauts-Bassins regions of Burkina Faso from June to November 2021.
End of Master perspective:
To set up a dengue surveillance program in Burkina Faso with a view to searching for other dengue serotypes (DENV 1, DENV3, DENV4) in mosquitoes and making surveillance data available to the Ministry of Health for better crisis management.
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Scientific publications :
- Mcclelland, "A worldwide survey of variation in scale pattern of the abdominal tergum of Aedes aegypti (L.) (Diptera: Culicidae)", 2009, doi: 10.1111/J.1365-2311.1974. TB00853. X.
- P. Pinheiro and S. J. Corber, "Global situation of dengue and dengue haemorrhagic fever, and its emergence in the Americas", World health statistics quarterly 1997; 50(3/4) : 161-169, 1997, Accessed: February 13, 2022. [Online]. Available at: https://apps.who.int/iris/handle/10665/55167.
- H. Kokernot, K. C. Smithburn, and M. P. Weinbren, "Neutralizing antibodies to arthropodborne viruses in human beings and animals in the Union of South Africa", J Immunol, vol. 77,no. 5, pp. 313-323, Nov. 1956.
- Le Goff et al, "Natural vertical transmission of dengue viruses by Aedes aegypti in Bolivia", Parasite, vol. 18,no. 3, pp. 277-280, August 2011, doi: 10.1051/parasite/2011183277.