The Scholars

of the WANIDA network

WANIDA > Scholars > Rejoice Ekli

Curriculum :
PhD in food safety

ACE affiliate :
ACEMFS, FUT, Minna, Niger State, Nigeria.

Thesis title :
Sequence identification of antibiotic resistance gene of Salmonella spp isolated from ready-to-eat meat in Accra, Ghana.

WANIDA > Scholars > Rejoice Ekli

Start date:
January 2021

Problem statement:
Rane (2011) reported that one of the most popular Ghanaian street foods, sold mainly at night, is ready-to-eat meat. Foodborne diseases are challenges in developing countries due to the inability to achieve standard hygienic food handling practices and poor sewage disposal (WHO, 2007). The increase in the development of antimicrobial-resistant foodborne pathogens is due to the high dose of clinically essential antimicrobials in human and veterinary medicine (Marshall and Levy, 2011). This is a major public health problem worldwide, as these foods are generally not annealed before consumption. Hence the major concerns about antibiotic resistance genes, the public health risks associated with foodborne pathogens and the risks associated with antibiotic-resistant pathogens.

Expected result:
The study aims to identify the antibiotic resistance gene of salmonella salmonella isolated from ready-to-eat meat in Accra-Ghana using the sequence-based identification technique. The objectives of the study were:
> Isolation and sequence-based identification of different strains of Salmonella from ready-to-eat meats and from humans in Accra, Ghana.
> Determination of the antibiotic resistance profile of different strains of Salmonella and characterization of antibiotic resistance genes.
> Assess the prevalence of antibiotic residues in ready-to-eat meats sold in Accra, Ghana.
> Determine the food safety knowledge of ready-to-eat meat sellers in Accra, Ghana.

Impact:

> Ready-to-eat meats and humans harboring strains of the species Salmonella strains will be known.
> The incidence of antibiotic residues in ready-to-eat meats will be established.
> The antibiotic resistance profile, antibiotic resistance genes and genetic relatedness of Salmonella present both in RTE meats and in humans will be revealed.

Supervisor :
Dr. Bala, Jeremiah David

Co-supervisors :
Salamatu M-Machunga, Cn Ezekiel and Prof. Adzitey Frederick.